| Module Leaders |
Professor Ed Stanley |
|
Professor Andrew Elefanty
|
Host Organisation
|
Monash Immunology and Stem Cell Laboratories (MISCL), Monash University
|
Module description
The purpose of this module is to generate genetically modified PSCs as tools for use in research and biotechnology. The laboratory has previously described the development of genetically tagged MIXL1GFP/w hESC lines that facilitate the analysis of differentiation outcomes towards mesendodermal progenitors (Davis et al, Blood, 2008). In addition, we have also generated the ErythRED line which enables the identification of cells expressing globin genes (Hatzistavrou et al, Nat Methods, 2009). These genetically tagged hESC lines have proven extremely useful in developing protocols for the generation distinct cellular subsets which are marked by expression of the reporter.
This module proposes to develop a further three lines that will facilitate the analysis of the stepwise differentiation of hESCs toward definitive haematopoietic and erythroid lineage cells.
Aims
The aim of this module is to develop a further genetically tagged reporter lines that will facilitate the analysis of the stepwise differentiation of hESCs toward definitive haematopoietic and erythroid lineage cells.
Module Leader biographies
Professor Ed Stanley was trained in the area of colony stimulating factors under the supervision of Dr. Ashley Dunn at the Ludwig Institute for Cancer Research. Following completion of his Ph.D., Professor Stanley was awarded a Human Frontiers in Science Organisation Fellowship prior to taking up a CJ. Martin Fellowship. These fellowships allowed him to relocate to the National Institute for Medical Research, Mill Hill, London, where he studied HOX gene regulation, before returning to the Walter and Eliza Hall Institute of Medical Research (WEHI). In 2000, he was appointed Head of the Embryonic Stem Cell laboratory at WEHI before moving, at the beginning of 2002, to the Centre for Early Human Development at the Monash Institute for Reproduction and Development.
Professor Andrew Elefanty trained as a physician in medical oncology and completed a PhD in leukaemogenesis under the supervision of Prof Suzanne Cory at the Walter and Eliza Hall Institute of Medical Research in 1992. He was awarded an NHMRC Neil Hamilton Fairley traveling fellowship and a Roche traveling fellowship from the RACP in 1993, taking up a position at the National Institute for Medical Research in London in the laboratory of Professor Frank Grosveld, studying aspects of blood cell development. He returned to the Hall Institute in 1995 as a Special Fellow, Head of the Developmental Haematopoiesis laboratory.
Professor Ed Stanley and Professor Andrew Elefanty jointly head the Embryonic Stem Cell Differentiation Laboratory at Monash University. The laboratory is focussed on hESC differentiation along mesodermal (blood, endothelium and heart) and endodermal (pancreas) lineages. Significant contributions to the field include the development of robust systems for the efficient differentiation of hESC, safe animal product free medium and genetically modified hESC lines. The major goals of our work are to understand human development, generate tools for drug discovery, and eventually to provide a source of cells for therapy.
Contact details
Professor Ed Stanley
Professor Andrew Elefanty
Selected publications
- Micallef SJ, Janes ME, Knezevic K, Davis RP, Elefanty AG and Stanley EG. Retinoic acid induces Pdx1-positive endoderm in differentiating mouse embryonic stem cells. Diabetes, 54, 301-5, 2005. (Citations = 32) (IF=7.9)
- Costa M, Dottori M, Ng ES, Hawes SM, Sourris K, Jamshidi P, Pera MF, Elefanty AG and Stanley EG. The hESC line Envy expresses high levels of GFP in all differentiated progeny. Nat Methods, 2, 259-260, 2005. (Citations = 29 (IF=14.9)
- Ng ES, Davis RP, Azzola L, Stanley EG and Elefanty AG. Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation. Blood, 106, 1601-3, 2005.(Citations = 43) (IF=10.1)
- Hirst CE, Ng ES, Azzola L, Voss AK, Thomas T, Stanley EG and Elefanty AG. Transcriptional profiling of mouse and human ES cells identifies SLAIN1, a novel stem cell gene. Dev Biol, 293, 90-103, 2006. (Citations = 10) (IF=4.8)
- Costa M, Dottori M, Sourris K, Jamshidi P, Hatzistavrou T, Davis R, Azzola L, Jackson S, Lim SM, Pera M, Elefanty AG and Stanley EG. A method for genetic modification of human embryonic stem cells using electroporation. Nature Protocols, 2, 792-796, 2007. (Citations = 11) (IF=1.5)
- Pick M, Azzola L, Mossman A, Stanley EG and Elefanty AG. Differentiation of human embryonic stem cells in serum free medium reveals distinct roles for BMP4, VEGF, SCF and FGF2 in hematopoiesis. Stem Cells, 2007 25(9):2206-14. (IF=7.9)
- Davis RP, Ng ES, Costa M, Mossman AK, Sourris K, Elefanty AG and Stanley EG. Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak-like cells and enables isolation of primitive hematopoietic precursors. Blood, 111, 1876-1884, 2008 (IF = 10. 896)
- Ng ES, Davis R, Stanley EG and Elefanty AG. A protocol describing the use of a recombinant protein-based, animal product free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies. Nature Protocols, 3, 768-776, 2008. (IF= 1.671)
- Davis RP, Costa M, Grandela C, Holland AM, Hatzistavrou T, Micallef SJ, Li X, Goulburn AL, Azzola L, Elefanty AG and Stanley EG. A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis. Nature Protocols, 3, 1550-1558, 2008. (IF= 1.671)
- Lim SM, Pereira L, Wong MS, Hirst CE, Van Vranken BE, Pick M, Trounson A, Elefanty AG and Stanley EG. Enforced expression of Mixl1 during mouse ES cell differentiation suppresses hematopoietic mesoderm and promotes endoderm formation. Stem Cells, 2008, in press. (IF= 7.531).