• Research Strategy
• Research Portfolio

• Platforms
• Programs
• Internal Research Groups

• Adult Lung Stem Cell Laboratory
• Renal Regeneration Laboratory
• Developmental Biology Laboratory
• Human Embryonic Stem Cell Technology
• Niche Laboratory
• Stem Cell Expansion Laboratory
• Tissue Repair

• External Research Groups
• Core Services

• Research Funding

• Development
• Donations to Science

Adult Lung Stem Cell Laboratory

Group Leader
Ivan Bertoncello MSc, PhD
Senior Scientist

Contact Details
Email: ivan.bertoncello@stemcellcentre.edu.au

To find out more about Ivan Bertoncello MSc, PhD, click here.

Lab Members

Jonathan McQualter - Post-Doctoral Research Scientist
Brenda Williams - Senior Research Assistant
Karen Yuen - Research Assistant
Rosa McCarty - Post-Doctoral Research Scientist

Research Focus

Isolation and characterization of stem cells in the adult lung.

Overview

Intractable, life-threatening respiratory diseases such as chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis are leading causes of mortality and morbidity worldwide and are currently treated by palliation with no prospect of cure. These diseases are prime candidates for stem cell based therapies. However, this objective has been hindered by the inability to identify endogenous lung stem cells around which these therapies can be developed, and by the lack of knowledge about the way in which adult lung stem cells interact with their stromal microenvironment and the biomatrix scaffold of the lung.

The broad objectives of this research program are to identify and characterize the respiratory epithelial and mesenchymal stem cell of the adult mouse lung, and to characterize the respiratory stem cell inductive microenvironments in which they reside and which specify their fate and regulate their regenerative potential.

Funding

NHMRC
Anti-Cancer Council of Victoria

Selected Publications

Wong SHA, Lowes KN, Bertoncello I, Cook MJ, Simmons PJ, Kornberg AJ, Kapsa RMI. Evaluation of Sca-1 and c-Kit as selective markers for muscle remodelling by non-hemopoietic bone marrow cells. Stem Cells. 2007; In Press

Haylock DN, Williams B, Johnston HM, Liu MCP, Rutherford KE, Whitty GA, Simmons PJ, Bertoncello I, Nilsson SK. HSC with higher hemopoietic potential reside at the bone marrow endosteum. Stem Cells. 2007; 25:1062-1069.

Wilson TJ, Lacham-Kaplan O, Gould J, Holloway A, Bertoncello I, Hertzog PJ, Trounson A. Comparison of mice born after intracytoplasmic sperm injection with in vitro fertilization and natural mating. Mol Reprod Dev. 2007; 74:512-519.

Nilsson SK, Simmons PJ, Bertoncello I. Hemopoietic stem cell engraftment. Exp Hematol. 2006; 34:123-129.

Challen GA, Bertoncello I, Deane JA, Ricardo SD, Little MH. Kidney side population reveals multilineage potential and renal functional capacity but also cellular heterogeneity. J Am Soc Nephrol. 2006; 17:1896-1912.

Nilsson SK, Johnston HM, Whitty GA, Williams B, Webb RJ, Denhardt DT, et al. Osteopontin, a key component of the hemopoietic stem cell niche and negative regulator of primitive hemopoietic progenitor cells. Blood. 2005; 106:1232-1239.

Sarsero JP, Holloway TP, Li L, McLenachan S, Fowler KJ, Bertoncello I, et al. Developmental and tissue specificity of FRDA gene expression in transgenic mice containing an FRDA-EGFP genomic reporter. Mamm Genome. 2005; 16:228-241.

Kaur P, Li A, Redvers R, Bertoncello I. Keratinocyte stem cell assays: an evolving science. J Investig Dermatol Symp Proc. 2004; 9:238-247.

Ramsay RG, Micallef SJ, Williams B, Lightowler S, Vincan E, Heath J, et al. Colony-stimulating Factor 1 promotes clonogenic growth of normal murine colonic crypt epithelial cells in vitro. J Interferon Cytokine Res. 2004; 24:416-427.

Ramsay RG, Micallef S, Lightowler S, Mucenski ML, Mantamadiotis T, Bertoncello I. c-myb heterozygous mice are hypersenstive to 5-fluorouracil and ionizing radiation. Mol Cancer Res. 2004; 2:354-361.

Bertoncello I, Williams B. Hematopoietic stem cell characterization by Hoechst 33342 and Rhodamine 123 staining. In: Hawley TS, Hawley RG, editors. Methods in Molecular Biology Vol 263: Flow Cytometry Protocols. 2nd ed. Totowa, New Jersey: Humana Press Inc; 2004. p. 181-200.

Bradford GB, Bertoncello I. Non-invasive measurement of hematopoietic stem cell cycling and turnover by in vivo bromodeoxyuridine incorporation. In: Klug CA, Jordan CT, editors. Methods in Molecular Medicine Vol 63: Hematopoietic Stem Cell Protocols. Totowa, New Jersey: Humana Press Inc; 2002. p.79-92.